Fig. 4

In vitro immune-regulated effect on macrophage polarization. A–B Cell endocytosis of MNV@DHA in RAW 264.7 cells. DiR-stained MNV@DHA (10 μg/mL of DHA) was incubated within RAW 264.7 cells for indicated time and examined by fluorescence microscope (A) and flow cytometry (B). C–D RAW 264.7 cells (M0) were incubated with different formulations (10 μg/mL of DHA) for 48 h and then stained with anti-CD86 for fluorescence microscope (C) and flow cytometric analysis (D). (E–F) RAW 264.7 cells were pretreated with IL-4 (20 ng/mL) for 48 h. The cells were rinsed with PBS for three times and then incubated with different formulations (10 μg/mL of DHA) for 48 h followed by staining with anti-CD206 for fluorescence microscope (E) and flow cytometric analysis (F)