Fig. 2

in vitro cellular uptake and endosomal escape of NPs measured by CLSM and flow cytometry. A The confocal fluorescence images of C6 cells incubated with DOX, DOX@NGs, or DOX@PNGs with the same DOX concentration of 5 μg/mL for 2 h; red = DOX, blue = nuclei; scale bars = 25 μm. B Flow cytometry analysis of C6 cells incubated with DOX, DOX@NGs, or DOX@PNGs with the same DOX concentration of 5 μg/mL for 2 h. C Quantification of the mean fluorescence intensities of the C6 cells. Data represent the means ± SD, **p < 0.01, ***p < 0.001. D The confocal fluorescence images of macrophage cells incubated with DOX, DOX@NGs, or DOX@PNGs with the same DOX concentration of 5 μg/mL for 2 h; red = DOX, blue = nuclei; scale bars = 50 μm. E Flow cytometry analysis of macrophage cells incubated with DOX, DOX@NGs, or DOX@PNGs with the same DOX concentration of 5 μg/mL for 2 h. F Quantification of the mean fluorescence intensities of the macrophage cells. Data represent the means ± SD, **p < 0.01, ***p < 0.001