Doxorubicin-loaded liposomes surface engineered with the matrix metalloproteinase-2 cleavable polyethylene glycol conjugate for cancer therapy

Table 1 Physicochemical characteristics of Dox-loaded liposomal formulations

Formulation	Lipid composition	Particle size (nm ± SD)^a	PDI^b ± SD	Zeta-potential (mV) ± SD	EE%^c
F1 (Caelyx^®)	HSPC/Chol/DSPE-PEG2000/α-tocopherol (56.7/38/5.3/0.2)	96.000 ± 2.12	0.10 ± 0.01	− 10.2 ± 0.38	100
F2	HSPC/Chol/DOPE-peptide-m-PEG/α-tocopherol (59.5/38/2.5/0.2)	159.501 ± 3.51	0.19 ± 0.02	− 7.61 ± 0.29	94
F3	HSPC/Chol/DOPE-peptide-m-PEG/α-tocopherol (57/38/5/0.2)	160.712 ± 1.92	0.18 ± 0.03	− 5.31 ± 0.31	90
F4	DOTAP/HSPC/DOPE/Chol/α-tocopherol (10/50/2/38/0.2)	127.173 ± 1.31	0.13 ± 0.01	+ 17.6 ± 0.22	88
F5	DOTAP/HSPC/Chol/DOPE-peptide-PEG/α-tocopherol (10/49.5/38/2.5/0.2)	130.931 ± 2.25	0.12 ± 0.04	+ 14.9 ± 0.13	95
F6	DOTAP/HSPC/Chol/DOPE-peptide-PEG/α-tocopherol (10/47/38/5/0.2)	124.702 ± 4.12	0.15 ± 0.02	+ 10.3 ± 0.41	84
F7	DOTAP/HSPC/DOPE/DSPE-PEG2000/Chol/α-tocopherol (10/47/2.5/2.5/38/0.2)	120.816 ± 3.19	0.10 ± 0.01	+ 13.6 ± 0.16	92
F8	DOTAP/HSPC/DOPE/DSPE-PEG2000/Chol/α-tocopherol (10/42/5/5/38/0.2)	170.312 ± 2.95	0.18 ± 0.04	+ 3.67 ± 0.34	87

^aDiameter of liposomes (Z-average)
^bPolydispersity index
^cEncapsulation efficiency. Values are presented from triplicate measurements of each formulation. Data are presented as mean ± standard deviation (SD)

ISSN: 1868-6966