Fig. 6
Immune response induced by MnO2-C/M-HA in vivo. a Photothermal images of tumor-bearing mice model. b The temperature changing curves of photothermal imaging in tumor site under laser treatment (660 nm, 1.0 W cm−2, 5 min). c Western blot results of CRT and HMGB1 expression in tumor tissues. I, Control; II, Control + laser; III, Free MTZ + Ce6 + laser; IV, MnO2-Ce6-HA + laser; V, MnO2-MTZ-HA + laser; VI, MnO2-C/M-HA; VII, MnO2-C/M-HA + laser. d-e Flow cytometry examined DC maturation levels and CD3+ CD8+ T cells in the tumor tissue of mice. f-g ELISA results of the level of the secreted TNF-α and IL-6 in blood serum after various treatments. All the data were displayed as mean ± SD (*p < 0.05, **p < 0.01 and ***p < 0.001)