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Fig. 3 | Cancer Nanotechnology

Fig. 3

From: Bufalin-loaded vitamin E succinate-grafted chitosan oligosaccharide/RGD-conjugated TPGS mixed micelles inhibit intraperitoneal metastasis of ovarian cancer

Fig. 3

Effect of BU@MMs on the proliferation and apoptosis of A2780 and SKOV3 cells. a, b The viability of A2780 and SKOV3 cells after incubation with BU, MMs and BU@MMs analyzed by CCK-8 assay. The apoptotic rates of A2780 (c) and SKOV3 (d) cells after incubation with BU, MMs, and BU@MMs evaluated by flow cytometry. Untreated cells were used as controls. Bar charts showing the average apoptotic ratio of A2780 (e) and SKOV3 (f) cells. Western blotting was performed to detect the protein expression of bcl-2, bax, cleaved caspase-3 and cleaved caspase-9 in A2780 (g) and SKOV3 (h) cells. The data are presented as the mean ± SD (n = 3). *P < 0.05, ***P < 0.001 indicate statistical significance compared with the control

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