Fig. 2

In vitro anti-tumor effect of Man-NPs. a Cytotoxicity study for K7 cells treated with drugs for 48 h. b Apoptosis assay of K7 cells via FACS. The right panel displayed the results of the apoptosis rate. c Western blotting was used to detect apoptotic protein. d VEGFA level of K7 cells after treatment. e Schematic of the transwell co-culture model. f FACS assay for measuring apoptosis in K7 cells co-cultured with TAM2. The right panel displayed the results of the apoptosis rate. g Western blotting was used to identify the apoptotic protein produced by K7 cells co-cultured with TAM2. h VEGFA levels in K7 cells co-cultured with TAM2. Comparisons were made using one-way ANOVA with Tukey’s test; ns: no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001