Fig. 3

In vitro control of macrophage repolarization. a Schematic illustration of BMDMs isolation, culture, and stimulation. b CD206 expression were detected by western blotting in TAMs after drug treatment. c Schematic of the transwell co-culture model. d The expression of CD206 in the drug-treated TAM2 co-cultured with K7 cells. The mRNA levels of CD86, TNF-α, CD206, and TGF-β in the drug-treated TAM1 (e) and TAM2 (f) co-cultured with K7 cells. g ELISA analysis of the amount of secreted IL-10 in the co-culture of K7 cells and TAM2 after drug treatment. Expression of p-STAT3 and NF-κB in TAM1 (h) and TAM2 (i) co-cultured with K7 cells after drug treatment. Comparisons were made using one-way ANOVA with Tukey’s test; ns: no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001