Fig. 7

In vivo remodeling TME. The percentage of TAMs with CD206⁺ (a) and CD86⁺ cells (b) was determined by FACS in subcutaneous tumor tissues. c The intratumoral ratio of CD86⁺ M1/CD206 + M2 phenotype. The percentage of CD8⁺ cells (d) and granzyme B⁺ CD8⁺ T cells (e) in intratumoral infiltration. The percentage of CD4⁺ cells (f) and Foxp3⁺ CD4⁺ T cells (g) in intratumoral infiltration. ELISA analysis of intratumoral cytokine secretion of IFN-γ (h) and TNF-α (i). j The expression of CD206, HIF-1α and VEGFR2 were detected by western blotting in subcutaneous tumor tissues after drug treatment. k Immunohistochemical staining (brown color) of CD86, CD86, and CD31 (scale bars: 100 µm). Comparisons were made using one-way ANOVA with Tukey’s test; ns: no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001