Fig. 2

T-MPs draw monocytes in blood to the vaccine injection site. a BALB/c mice (n = 3) were intramuscularly immunized in the right rear thigh with H22-MPs and in the left rear thigh with PBS. After 48 h, mice were sacrificed, and monocytes in muscles around the femur were measured by flow cytometry. b Similar setting as in a and the thigh muscle tissues were removed for paraffin sections and immunofluorescence staining, then observed by a two-photon confocal microscope. c BALB/c mice were injected with 1 × 10⁶ PKH26-stained allogeneic monocytes by tail vein, followed by inoculation with H22-MPs via i.m. injection. After 48 h, recruitment of PKH26⁺ monocytes was analyzed via flow cytometry. Mean ± s.e.m. is represented in the data and two-tailed unpaired Student's t test was used to statistically analyze the P value. **P < 0.01