Fig. 4

Monocytes that have endocytosed T-MPs enter dLN and differentiate into moDCs. a–d BALB/c mice (n = 4) were immunized in the right rear thigh with H22-MPs and in the left rear thigh with PBS via i.m. injection. After 48 h, mice were sacrificed, and inguinal lymph nodes of both legs were isolated which were taken picture with a digital camera (a). DCs (b), CD8⁺IFNγ⁺ T lymphocytes (c) and moDCs (d) in inguinal lymph nodes were measured via flow cytometry. e Wild-type (WT) C57BL/6 mice and CCR2^−/− mice were intramuscularly injected with B16-MPs. After 48 h, monocytes in thigh muscles were measured by flow cytometry. f WT C57BL/6 mice and CCR2^−/−mice were immunized in the right rear thigh with B16-MPs and in the left rear thigh with PBS via i.m. injection. After 48 h, mice were sacrificed, and moDCs in inguinal lymph nodes were measured via flow cytometry. g CCR2^−/− mice immunized with B16-MPs or PBS were subjected to an i.m. injection of 5 × 10⁵ B16-F10 melanoma cells on day 0. The tumor weight was measured on day 10. h Splenic CD8⁺ T lymphocytes derived from OT-1 mice were co-incubated with CD11c⁺MHCII⁺CD64⁻ DCs (Ctrl) or CD11c⁺MHCII⁺CD64⁺ moDCs from OVA-MP immunized C57BL/6 mice. T-lymphocyte proliferation was measured using flow cytometry. i The expression of the H-2 Kb-OVA257-264 peptide complex in CD11c⁺MHCII⁺CD64⁻ DCs (Ctrl) and CD11c⁺MHCII⁺CD64⁺ moDCs was measured via flow cytometry. Mean ± s.e.m. is represented in the data and two-tailed unpaired Student's t test was used to statistically analyze the P values. **P < 0.01; ***P < 0.001; ****P < 0.0001; NS, not significant