Fig. 1
Cytotoxicity of Nano particles on HBE cells. A. Characteristics of Nano-ZnO and Nano-SiO2 by Transmission Electron Microscope (TEM). B. Cells were seeded in 96-well plates. After overnight culture, cells were treated with different concentration of nano particles for 24 h. Cells without nanoparticles treatment were used as the control. Cytotoxicity was determined by CCK8. C. The 5-Ethynyl–2ʹ-deoxyuridine (EdU) positivity of HBE cells after different nanoparticles concentration treatment. Scale bar = 50 µm. Data were expressed as the mean ± SD. Differences between two groups were analyzed by t-test. When there were more than two groups, one-way analysis of variance (ANOVA) followed by Dunnett’s post-hoc test was used for comparisons with the control. If there were two independent variables on a dependent variable, two way ANOVA followed by Holm-Sidak post-hoc test was employed. If necessary, transformation of data was used to achieve normally distributed data before analysis. A difference was considered statistically significant when a p-value was less than 0.05