Fig. 4

Silencing p53 promoted circRNA_0085439 translocation from cytoplasm to nucleus after Nano-ZnO treatment. A. For left gel, the first 1 lane presents the RNA product fraction of circRNA_0085439 between 200 and 500 bp; while the second lane presents the size of production is between 700 and 1000 bp. For right gel, divergent primers were used to amplify circRNA_0085439 from cDNA but not genomic DNA (gDNA). Convergent primers amplified both of circRNA_0085439 and the linear GAPDH RNA in cDNA and gDNA. B. qRT-PCR was conducted to detect the circRNA_0085439 relative expression at indicated time points using μg/mL of Nano-ZnO. C and in both of p53 WT cells and p53-KO cells after different Nano-ZnO concentrations treatment (D). E. Representative images of circRNA_0085439 expression in p53 WT cells and p53-KO cells post 30 μg/mL of Nano-ZnO for 24 h analyzed by immunofluorescence. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Data were expressed as the mean ± SD. Differences between two groups were analyzed by t-test. When there were more than two groups, one-way analysis of variance (ANOVA) followed by Dunnett’s post-hoc test was used for comparisons with the control. If there were two independent variables on a dependent variable, two way ANOVA followed by Holm-Sidak post-hoc test was employed. If necessary, transformation of data was used to achieve normally distributed data before analysis. A difference was considered statistically significant when a p-value was less than 0.05