Fig. 6

In vivo abscopal effect of RT combined with R837@PLGA or R837@PLGA@Neu in bilateral CT26 tumor-bearing mice. A Schematic illustration of combination therapy in vivo. B Tumor growth curves of right irradiated tumors after various treatments. C Tumor growth curves of left nonirradiated tumors and body weights of mice after various treatments. D Representative images of TUNEL staining in nonirradiated tumor tissues after treatments (scale bar: 50 μm). E Quantification of TUNEL-positive cells in tumor sections. F IHC staining of Ki67 in nonirradiated tumor tissues after treatments (scale bar: 50 μm). G Proportions of Ki67-positive cells in tumor sections. H Proportion of CD8⁺ T cells in CD45⁺ cells and ratio of CD8⁺/CD4⁺ cells in nonirradiated tumors by flow cytometry. Numerical data are presented as the mean ± SD. *P < 0.05; **P < 0.01