Fig. 5
The effects of ZnO NCs and Fe:ZnO NCs incubation and single SW treatment in A HT-29 and B Colo 320DM cells. Both cell lines were incubated with 10 µg/ml of NCs in 96-well plates in replicates. After 24 h of NCs incubation, cells were exposed to 250 shots of SW (0.12 mJ/mm.2, 4 shots/s). Control experiments were also carried out for comparison. CT represents untreated control cells; NCs are cells exposed only to ZnO NCs or Fe:ZnO NC; SW represents cells exposed to SW; NCs + SW are cells subjected to the combined treatment. After 24, 48 and 72 h, the cell viability was recorder by the WST-1 assay. Data are expressed as mean of cell viability ± SEM. n ≥ 3. Statistical relevance is indicated in comparison with control sample (*p < 0.05, **p < 0.01, ***p < 0.001)