Fig. 6
The effects of ZnO NCs and Fe:ZnO NCs ZnO NCs incubation and 3SW treatments in A HT-29 and B Colo 320DM cells. Both cell lines were incubated with 10 µg/ml of NCs in 96-well plates in replicates. After 24 h of NCs incubation, wells were exposed to 250 shots of SW (0.12 mJ/mm.2 4 shots/s) for three times (a treatment every 4 h), while control cells remained untreated or received only the NCs or 3SW treatments. CT represents untreated cells; NCs are cells exposed only to ZnO NCs or Fe:ZnO NC; 3SW represents cells exposed to SW for three times; NCs + 3SW are cells subjected to the combined treatment. After 24, 48 and 72 h, the cell viability was recorder by the WST-1 assay. Data are expressed as mean on cell viability with relative to untreated cells ± SEM. n ≥ 3. Statistical relevance is indicated in comparison with control sample (*p < 0.05, **p < 0.01, ***p < 0.001)