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Table 4 Composition of PTS-loaded EN according to 23 Factorial design

From: Formulation and optimization of folate-bovine serum albumin-coated ethoniosomes of pterostilbene as a targeted drug delivery system for lung cancer: In vitro and in vivo demonstrations

Formula

Variables

Independent Variables

Dependent Variables

X1

X2

X3

Y1*

Y2*

F1

− 1

1

1

94.61 ± 0.90

71.22 ± 0.25

F2

− 1

1

− 1

91.00 ± 0.63

85.83 ± 0.55

F3

1

1

1

95.31 ± 3.94

74.70 ± 0.71

F4#

1

1

− 1

93.80 ± 0.90

74.85 ± 1.15

F5

− 1

− 1

1

95.96 ± 3.61

52.71 ± 0.61

F6

− 1

− 1

− 1

92.81 ± 1.80

71.38 ± 0.49

F7

1

− 1

1

96.60 ± 2.39

63.06 ± 0.36

F8

1

− 1

− 1

94.77 ± 3.25

67.5 ± 0.353

Independent variables

Low (− 1)

High (+ 1)

X1: Amount of span 60

X2: Amount of ethanol

X3: Amount of CHO

25 mg

10%

25 mg

100 mg

20%

50 mg

  1. Q24h, percentage of PTS released after 24 h; EE, entrapment efficiency
  2. #optimized Formula, * the data are expressed as mean ± SD (n = 3); all formulations included 10 mg PTS, Y1: EE (%), Y2: Q24h (%)