Fig. 4

a Confocal fluorescence imaging of B16 F10 cells after staining with the APD–PD-L1–MIPL, MIP and NIP. The APD–PD-L1–MIPL, MIP, NIP and nuclei are visualized in green and blue, respectively. b, c Proliferation of T lymphocytes in different groups after staining with CFDA-SE. d Imaging of apoptosis of B16 F10 cells after staining with Hoechst 33,258 (blue). The concentration of the APD–PD-L1–MIPL, MIP and NIP was 0.6 mg mL⁻¹, respectively