Fig. 5

ADAMTS9-AS1 affects the mRNA stability of ADAMTS9 in ccRCC. A, C. The mRNA expression levels of ADAMTS9 were detected by qRT-PCR in A498 and OS-RC-2 cells following ADAMTS9-AS1 overexpression (A) and KD (C). B, D. The protein expression levels of ADAMTS9 were detected by WB in A498 and OS-RC-2 cells following ADAMTS9-AS1 overexpression (B) and KD (D). (E). The expression levels of ADAMTS9 and ADAMTS9-AS1 were detected by qRT-PCR in an ADAMTS9-AS1 overexpression xenograft model. (F). The protein expression levels of ADAMTS9 were detected by WB in the ADAMTS9-AS1 overexpression xenograft model. G-H. The mRNA (G) and protein (H) expression levels of ADAMTS9 were detected by qRT-PCR in ADAMTS9-AS1 knockdown xenografts with or without 5-aza-dC treatment. I. ADAMTS9 promoter luciferase reporter activity was detected by luciferase reporter assay after ADAMTS9-AS1 overexpression or KD. J. The half-life of ADAMTS9 mRNA was measured by qRT-PCR in the presense of ActD treatment in A498 (left) and OS- RC-2 (right) cells. The data derived from three independent experiments are presented as the mean ± SEM in the bar graphs (A, C, E, G, I, J). Controls were normalized to 1 (A, C, E, G, I, J). **P < 0.01, ***P < 0.001. N.S. not significant