Fig. 8

The ADAMTS9-AS1/ADAMTS9 axis inhibits ccRCC cell growth and drug resistance. A-B. The mRNA expression levels of ADAMTS9 were detected by qRT-PCR in ccRCC tissues (n = 50) (A) and ccRCC cell lines (B). C-D. ADAMTS9 mRNA and ADAMTS9-AS1 expression levels showed a positive linear correlation in ccRCC tissues (C) and TCGA database (D). E-F. A498 and OS-RC-2 cell viability was detected by CCK-8 assay after ADAMTS9-AS1 overexpression or ADAMTS9 knockdown with (F) or without (E) 5-aza- dC treatment. G A498 and OS-RC-2 apoptosis rates were detected by FACS following ADAMTS9-AS1 overexpression or ADAMTS9 knockdown with or without 5-aza-dC. The data derived from three independent experiments are presented as the mean ± SEM in the bar graphs (B, E, F, G). Controls were normalized to 1 (B, E, F, G). *P < 0.05, **P < 0.01, ***P < 0.001