Table 1 Primer’s sequences utilized for RT-qPCR
Genes | Sequence |
|---|---|
Akt | Forward: 5′-CTGCTCAAGAAGGACCCCAA-3′ |
Reverse: 5′-AGGTGGTGTGATGGTGATC-3′ | |
mTOR | Forward: 5′-CCAGGAGTTACTTCTATGCC-3′ |
Reverse: 5′-GAAATCCGCTTGTTAGGGTC-3′ | |
NF-κB | Forward: 5′-ACACCGAAGCAATTGAAGTG-3′ |
Reverse: 5′-ATGGGGCATTTTGTTGAGAG-3′ | |
CDK2 | Forward: 5′-GAGCCAGCCCATAAGAAAAC-3′ |
Reverse: 5′-CACCAGCCAATATAGCACTG-3′ | |
Rb | Forward: 5′-GAATCATTCGGGACTTCTG-3′ |
Reverse: 5′-TCTGCTTCATCTGATCCTTC-3′ | |
GAPDH | Forward: 5′-CAAGTTCAACGGCACAGTCAAG-3′ |
Reverse: 5′-CATACTCAGCACCAGCATCACC-3′ |