Fig. 4From: Nanoliposomal VEGF-R2 peptide vaccine acts as an effective therapeutic vaccine in a murine B16F10 model of melanomaAnalysis of the frequency of T cell subpopulations. Isolated CD8+ T cells A, CD4+ T cells B, and CD25+ FoxP3+ Treg cells C from spleen of vaccinated mice. On day 30th post final vaccination, three mice per group were sacrificed. Splenocytes were isolated and stimulated in vitro with V1, V2, and V3 peptides. Flow cytometry was used to study cells labeled with fluorescently tagged antibodies. Data are presented as mean standard deviation (n = 3). Lip Liposome; P > 0.05; *, P < 0.05; **, P < 0.01; *** P < 0.001, ****P < 0.0001Back to article page