Fig. 5

Mean fluorescence intensity (MFI) of intracellular cytokines. On the day 30th post final vaccination, three mice per group were sacrificed, and splenocytes were isolated and stimulated with V1, V2, and V3 peptides. Then the cells were stained with antibodies targeting intracellular cytokines. Flow cytometry was used to measure the MFI of various cytokines. The intracellular cytokines in CD8⁺ and CD4⁺ splenocytes were measured. This involved assessing the geometric mean fluorescence intensity of IFN-γ in CD8⁺ cells, IFN-γ in CD4⁺ cells, IL-4 in CD4⁺ cells, and IL-10 in CD4⁺ Foxp3⁺ cells of mice that had received therapeutic vaccines. Data are shown as mean ± standard deviation (n = 3). Lip, Liposome, *P > 0.05, **P < 0.05, ***P < 0.01, ****P < 0.001, *****P < 0.0001