Fig. 3

a ROS production level of Sk-Br3, MCF-7, U87, and HF-5 after 4 h incubation with 10 µM Ce6 followed by irradiation using 665 nm for various durations expressed as fold difference relative to the control. b Apoptotic and necrotic % of Sk-Br-3, U87, and HF-5 cells after treatment with 10 µM Ce6-PDT conditions corresponding to IC50 conditions. c Apoptotic and necrotic % of Sk-Br-3, U87, and HF-5 cells after treatment with 0.6 mM 5-ALA-PDT conditions corresponding to IC50 conditions. d Caspase 3/7 level of Sk-Br-3, MCF-7, and U87 cells after treatment with 10 µM Ce6-PDT conditions corresponding to IC50 conditions. e Caspase 3/7 level of Sk-Br-3, MCF-7, and U87 cells after treatment with 0.6 mM 5-ALA-PDT conditions corresponding to IC50 conditions. Treatment conditions are summarized in Table 3. All experiments were repeated at least 3 times and presented are mean and standard deviation