Fig. 2

Ultrastructure of mitochondria and regulation of key proteins associated with ART-induced ferroptosis. A Representative transmission electron microscope photographs of mitochondria in HepG2 and Huh7 cells treated for 24 h with 0 or 80 µM ART (scale bar: 500 nm). B Representative immunoblots and C graphic representation of relative expression of TFRC, SLC7A11, GPX4, FTH1 and Tubulin in HepG2 and Huh7 cells exposed to 0, 20, 40, 80 µM ART for 24 h. (n = 3 per group, *P < 0.05, **P < 0.01, ***P < 0.001). Bars indicate mean ± SEM