Fig. 7

MSN-ART and MSN-ART-FA enhanced ART-induced ferroptosis In vivo. A Image of tumor tissues after HepG2 tumor-bearing mice were killed. B Relative tumor volumes during treatment of HepG2 tumor-bearing mice that received saline, ART, MSN-ART or MSN-ART-FA treatments. (n = 4 per group, *P < 0.05). C H&E images of tumor tissues (scale bar: 50 μm). D Fluorescence signal intensity of ROS in tumor tissues. E ROS level, F iron content, G MDA content and H GSH content in tumor tissues of HepG2 tumor-bearing mice that received saline, ART, MSN-ART or MSN-ART-FA treatments. (n = 3 per group, *P < 0.05). I Representative immunoblots and J graphic representation of relative expression of TFRC, SLC7A11, GPX4, FTH1 and Tubulin in tumor tissues of HepG2 tumor-bearing mice that received saline, ART, MSN-ART or MSN-ART-FA treatments. (n = 3 per group, *P < 0.05, **P < 0.01, ***P < 0.001 compared to control, #P < 0.05, ##P < 0.01 compared to ART, $P < 0.05). Bars indicate mean ± SEM