Fig. 4

In vivo IFN-γ secretion induced by PD-1 NVs and pro-ferroptosis effect in breast cancer cells. a Serum IFN-γ level in mice bearing 4T1 xenografts five times after PD-1 NVs treatment. b IFN-γ exacerbated the ferroptosis of 4T1 cells induced by PD-1@RSL3 NPs (IFN-γ: 100 ng/ml; RSL3: 5 μM). c Lipid peroxidation analysis in 4T1 cells treated with IFN-γ and different formulations via MDA assay (IFN-γ: 100 ng/ml; RSL3: 5 μM). d ROS production analysis in 4T1 cells treated with IFN-γ and different formulations (IFN-γ: 100 ng/ml; RSL3: 5 μM)