Fig. 6

Tumor tissue staining showed that PD-1@RSL3 NPs inhibited tumor growth and enhanced tumor immunogenicity in mice bearing breast cancer xenograft. a H&E staining of tumor tissues. Scale bar: 100 µm. b Ki67 staining indicated delayed tumor growth in mice treated with PD-1@RSL3 NPs. Scale bar: 100 µm. c Calreticulin (CRT) exposure analysis suggested that PD-1@RSL3 NPs significantly enhanced the ICD-associated immunogenicity. d Serum IFN-γ analysis in mice. e TNF-α analysis in different groups. P values are from one-way ANOVA followed by Tukey’s post-test (d, e)