Fig. 3

Relationship between EMT and apoptosis, MDR of MCF-7/ADR cell. A Detection of cell numbers at different times of apoptosis; B Apoptosis rates of each group; Annexin V-/PI- represents normal cells (a), Annexin V-/PI + represents naked nuclei or mechanically damaged cells (b), Annexin V + /PI- represents early apoptotic cells (c), and Annexin V + /PI + represents late apoptotic cells or necrotic cells (d). The cell apoptosis rate was defined as: (c + d)/(a + b + c + d) *100%. C Representative western blot bands of apoptosis related proteins; D The relative expression amounts of apoptosis related proteins; E Representative western blot bands of MDR related proteins; F Relative expression amounts of MDR related proteins. The numerical data (B, D, F) were presented as the mean ± SD (n = 3). One-way ANOVA followed by the Bonferroni post hoc test (B, D, F) were used for statistical significance analysis. All experiments were repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.