Fig. 6

E-PLNs reversed IL-6 induced apoptosis resistance of MCF-7/ADR cells. A-D. Cells were pretreated with IL-6 or AG490, and the percentage of apoptotic cells was determined by flow cytometry; Annexin V-/PI- represents normal cells (a), Annexin V-/PI + represents naked nuclei or mechanically damaged cells (b), Annexin V + /PI- represents early apoptotic cells (c), and Annexin V + /PI + represents late apoptotic cells or necrotic cells (d). The cell apoptosis rate was defined as: (c + d)/(a + b + c + d)*100%. E–F. Cells were pretreated with IL-6 or AG490, and the expression of apoptosis related proteins was determined by western blot. The numerical data (C, D, F, H) were presented as the mean ± SD (n = 3). One-way ANOVA followed by the Bonferroni post hoc test (C, D, F, H) were used for statistical significance analysis. All experiments were repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.