Fig. 1

Differential expression of MSLN in MPM subtypes. A Western blot and quantitative expression of MSLN in different MPM cells (H226, H2452, and MSTO-211H), MeT-5A, and A549 cells relative to β-actin. *p < 0.05, **p < 0.01, vs. MeT-5A. B mRNA expression of MSLN in the same cell lines as described in B, with GAPDH being the internal control. **p < 0.01, vs. other cells. C Western blot and quantification of MSLN in tumor tissues of tumor-bearing nude mice at 14, 28, and 42 days after molding, with β-actin being the loading control. D IHC staining for MSLN in tumor tissues of A549, MSTO-211H, and H226 cell lines, scale bar: 50 μm. E ELISA for SMRP in tumor-bearing mouse serum. Data are represented by means ± SEM. One-way ANOVA with Tukey’s multiple comparisons test (A, B, C, E). *p < 0.05; **p < 0.01