Fig. 2

The histological subtype of MPM is closely associated with cell migration, invasion, and survival. A Cell migration analysis of MeT-5A and MPM cells (H2452, H226, and MSTO-211H) at 0 h, 6 h, 12 h, 24 h, and 36 h in the scratch assay. B Migration rate line graph at 6-0 h, 12-0 h, 24-0 h, and 36-0 h. *p < 0.05, **p < 0.01, vs. 6–0 h. C-D Representative images (C) and quantitative analysis (D, migratory cells and crystal violet assays with OD values at 570 nm [OD_570nm]) of MPM cell and MeT5A cell migration were detected by Transwell migration assay. E, F Representative images (E) and quantitative analysis (F, invasive cells and crystal violet staining with OD570nm) of MPM cells and MeT5A invasion from Matrigel-coated Transwell. *p < 0.05, **p < 0.01, vs. MeT-5A; #p < 0.05, ##p < 0.01, vs. H226. G, H Tumor growth curves (G) and survival curves (H) in MSTO-211H and H226 tumor-bearing nude mice. Scale bar: 50 µm. One-way ANOVA with Tukey’s multiple comparisons test (B, D, F)