Fig. 3

Upregulation of MSLN promotes the expression of N-Cadherin, MMP7, and MMP9. A Western blot assay and relative expression of MSLN among NC, empty vector control (LV-NC), and MSLN overexpression (LV-MSLN) groups. B MSLN mRNA expression in NC, LV-NC, and LV-MSLN cells. ***p < 0.05, vs. NC; ###p < 0.01, vs. LV-NC. C Protein levels of N-Cadherin, MMP9, and MMP7 in H226 cells were markedly enhanced in the LV-MSLN group. D Quantitative analysis of N-Cadherin, E quantitative analysis of MMP9, F quantitative analysis of MMP7. GAPDH and β-actin served as internal controls in these assays. **p < 0.01, vs. LV-MSLN. One-way ANOVA with Tukey’s multiple comparisons test (A, B, D, E, F)