Fig. 5
Characterization and properties of the FSPM. XPS spectra of FSP (A) and (B) FSPM. C TEM images of the FSPM. D T2 relaxation rate (R2) and T1 relaxation rate (R1) of FSPM as a function of iron concentration. E Prussian blue staining showed iron-containing cells (black arrow), scale bar: 20 μm. F Quantitative analysis of Prussian-stained cell areas. SD: Standard deviation (*p < 0.05; **p < 0.01). G Fe3+ distribution in heart, lung, liver, spleen, kidney, and tumor tissues after injection of FSP and FSPM. *p < 0.05, vs. FSP. H Cell viability of A549 and H226 cells after incubation with FSPM at different concentrations. I Sections of the head, liver, lung, spleen, heart, and kidney, after injection of saline and FSPM (H&E stain 10 × magnification, scale bars: 50 μm). Abbreviation: FSP, Fe3O4@SiO2-PEG; FSPM, Fe3O4@SiO2-PEG-MSLN. One-way ANOVA with Tukey’s multiple comparisons test (F, G, I)