Fig. 2

Detection of pro-apoptotic molecules on U87-MG 3D spheroids treated with Pt-8AQ. A 2D versus 3D expression of BCL-2, PUMA, NOXA and BAX after 24 h of treatment with Pt-8AQ 5 µM (2D) or 20 µM (3D). The values are expressed as mean of two replicates. B RTq-PCR of proapoptotic molecules Caspase-3 on untreated U87-MG spheroids (CTRL) or treated 24 h with Pt-8AQ 5 µM. The values are expressed as mean of three replicates ± SEM. C Caspase-3 activity measurement after Pt-8AQ 5 µM treatment of spheroids (24 h). The histogram represents the optical density (OD) at 405 nm. Data are expressed as mean ± SEM of four replicates. (*p < 0,001)