Fig. 3

In vitro activity and cell-cycle effects of Pt-8AQ on adipose tissue-derived AT-MSCs. A Cytotoxic activity of Pt-8AQ was evaluated after 24, 48 and 72 h of treatment with different concentrations of fresh drugs (from 5 to 20 µM) and expressed as cell viability (% of control cells). The table reports the IC₅₀ values expressed as mean ± standard error (SEM) of six independent experiments. B Effect of increasing concentrations of Pt-8AQ was evaluated by a 7 day antiproliferation MTT assay on AT-MSCs. The effect was expressed as a percentage of the optical density measured in cultures that did not receive drugs (100% proliferation). C Histogram shows the effect of Pt-8AQ treatment (10 µM and 20 µM) on AT-MSCs after 24 h of treatment. The percentages of cells counted in each different cell phase (sub G0/G1, G0/G1, S and G2/M) are reported and compared to those found in untreated cells (CTRL). The values are expressed as mean ± standard error of five independent experiments. Two donors of AT-MSCs were used for the experiments. D Example of cell cycle analysis profile by flow cytometry