Fig. 5

Antitumoral effect of MVs secreted by AT-MSCs loaded with Pt-8AQ. A In vitro antiproliferative activity on U87-MG cell line of fresh drug, secretome, and MVs pellet isolated by ultracentrifugation (100000 g). The supernatant after centrifugation was also tested. Data are expressed as percentage of cell growth normalized on the effect of secretome, pellet, and supernatant obtained by untreated AT-MSCs. Dotted lines represent linear regression of each curve. Data are reported as the mean ± standard error of at least three independent experiments. B Number of MVs per cell counted in the secretome derived from Pt-8AQ-loaded AT-MSCs or control AT-MSCs. Data are reported as the mean ± standard error of at least three independent experiments. C and D Representative plot of size distribution analysis of the secretomes of AT-MSC©TRL C and AT-MSCs/Pt-8AQ D analyzed by nanoparticle tracking assay (NTA). In both samples, several populations of vesicles were present at 200−300 nm