Skip to main content

Table 3 Expression of genes involved in apoptosis was performed by real-time polymerase chain reaction (PCR)

From: Investigation of mitochondria-dependent apoptosis pathway and lipid peroxidation level induced by biosynthesized silver nanoparticles: caspase-3 activation, BAK1/BCLx regulation and malondialdehyde production

Genes

Sample

CT (mean)

ΔCT

ΔΔCT

RQ

BCLx

Control

15.5125

− 3.5735

0

1

AgNPs

32.3232

− 1.4181

2.1554

0.2245

BAK1

Control

26.1379

7.0518

0

1

AgNPs

35.7057

1.9643

− 5.0875

34.001

Caspase-3

Control

19.9565

0.8705

0

1

AgNPs

30.7537

− 2.9876

− 3.8581

14.501

GAPDH†

Control

19.086

   

AgNPs

33.7413

   
  1. For this purpose, the Applied Biosystems StepOne™ instrument (USA) was used. RQ (relative quantification) was considered equal to \({2}^{-\Delta \Delta CT}\). ΔΔCT is comparative cycle threshold. †GAPDH is an endogenous control and used to normalize data from other genes
Back to article page

Cancer Nanotechnology

ISSN: 1868-6966

Contact us