Fig. 5

RT-qPCR analysis, CTCF evaluation of the fluorescence microscopy images of MCF-7/GFP and cell viability assay. a copGFP gene expression in different conditions tested at 6 h and 24 h. Gene expression levels were normalised to the cells without anti-copGFP (Control). Black asterisks denote statistical differences between samples and the Control (*p < 0.05; **p < 0.01; ***p < 0.001; two-way ANOVA—Mixed-effects analysis with Tukey’s multiple comparison test). Data represents the mean value ± the standard error mean of at least three biologically independent experiments with two technical replicates for each. b CTCF analysis to evaluate copGFP protein expression at 6 h and 24 h post-transfection. Statistical differences were not observed between all the samples tested with control without anti-copGFP (Control). Data represent the mean value ± the standard error mean of two biologically independent experiments with three technical replicates each. c Cell viability analysis of the different samples tested after 6 h and 24 h of copGFP silencing via MTS assay. The three conditions tested were normalised to control. Statistical differences were observed between the cells treated with lipofectamine and cells without anti-copGFP (Control). Data represent the mean value ± the standard error mean of at least three biologically independent experiments with two technical replicates each