Fig. 6

RT-qPCR analysis and CTCF evaluation of c-MYC protein expression by Immunofluorescence assay of HCT116 2D cells. a c-MYC gene expression at 9 h of gene silencing. Gene expression levels were normalised to respective Controls. Black asterisks indicate statistical differences between samples and Control (**p < 0.01; ns—not statistically significant; Unpaired parametric t test with Welch’s correction). Data represent the mean value ± the standard error mean of at least three biologically independent experiments with two technical replicates each. b CTCF analysis via Immunofluorescence assay to evaluate c-MYC protein expression at 9 h of gene silencing. Black asterisks indicate statistical differences between the samples and Control (*p < 0.05; **p < 0.01; Unpaired parametric t test with Welch’s correction). Data represents the mean value ± the standard error mean of two biologically independent experiments with three technical replicates each. c Fluorescence microscopy images of 9 h after silencing experiment in Control, AuNP@c-MYC + Laser, and AuNP@c-MYC samples. Scale bars correspond to 20 μm