Fig. 7

c-MYC gene silencing in 3- and 7-day HCT116 3D spheroids. c-MYC gene expression at 9 h of gene silencing in a 3-day spheroids and b 7-day spheroids. Gene expression levels were normalised to the respective Controls. Black asterisks indicate statistical differences between samples and the Control (*p < 0.05; ns not statistically significant; Unpaired parametric t test with Welch’s correction). Data represents the mean value ± the standard error mean of at least three biologically independent experiments with two technical replicates for each