Fig. 8

CTCF evaluation of c-MYC protein expression in 3- and 7-day HCT116 spheroids. CTCF analysis of Immunofluorescence assay evaluates c-MYC protein expression at 9 h after gene silencing in (a) 3-day spheroids and (b) 7-day spheroids. Statistical differences were not observed between the samples tested with Control (ns not statistically significant; Unpaired parametric t test with Welch’s correction). Data represent the mean value ± the standard error mean of two biologically independent experiments with three technical replicates for each. c Fluorescence microscopy images of Control, AuNP@c-MYC, and AuNP@c-MYC + Laser sample in 3-day spheroids. Scale bars correspond to 300 μm. d Same as for c) in 7-day spheroid