Fig. 4

Therapeutic outcome and safety evaluation in orthotopic liver tumor-bearing mice. A Protocol for therapeutic outcome and safety evaluation. B PTX content in orthotopic liver tumor after drug treatment followed by HBO or HH exposure for 1.5 h. C Survival monitoring. D Photographs of harvested tumor-bearing livers. Red circles indicated tumor sites of the livers. E Liver weight index. F H&E staining of orthotopic liver tumor. Hematologic examination for (G) AST and (H) ALT reflecting liver function. I Hematologic examination for CK reflecting heart function. Orthotopic liver tumor-bearing mice were intravenously injected with PTX-LPs (5 mg PTX/kg body weight) and immediately subjected to HH or HBO exposure for 1.5 h. Data were presented as mean ± SEM (n = 3 or 8). ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001