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Lipid polymeric nanoparticles modified with tight junction-modulating peptides promote afatinib delivery across a blood–brain barrier model



Brain metastases from non-small cell lung cancer (NSCLC) remain one of the most challenging malignancies. Afatinib (Afa) is an orally administered irreversible ErbB family blocker approved for epidermal growth factor receptor (EGFR)-mutated NSCLC. However, the incidence of brain metastases in patients with NSCLC and EGFR mutation is high. One of the major obstacles in the treatment of brain metastases is to transport drugs across the blood–brain barrier (BBB). A lipid polymeric nanoparticle (LPN) modified with a tight junction-modulating peptide is a potential formulation to deliver therapeutics across the BBB. FD7 and CCD are short peptides that perturb the tight junctions (TJs) of the BBB. In this study, the use of LPN modified with FD7 or CCD as a delivery platform was explored to enhance Afa delivery across the BBB model of mouse brain-derived endothelial bEnd.3 cells.


Our findings revealed that Afa/LPN-FD7 and Afa/LPN-CCD exhibited a homogeneous shape, a uniform nano-scaled particle size, and a sustained-release profile. FD7, CCD, Afa/LPN-FD7, and Afa/LPN-CCD did not cause a significant cytotoxic effect on bEnd.3 cells. Afa/LPN-FD7 and Afa/LPN-CCD across the bEnd.3 cells enhanced the cytotoxicity of Afa on human lung adenocarcinoma PC9 cells. FD7 and CCD-modulated TJ proteins, such as claudin 5 and ZO-1, reduced transendothelial electrical resistance, and increased the permeability of paracellular markers across the bEnd.3 cells. Afa/LPN-FD7 and Afa/LPN-CCD were also partially transported through clathrin- and caveolae-mediated transcytosis, revealing the effective activation of paracellular and transcellular pathways to facilitate Afa delivery across the BBB and cytotoxicity of Afa on PC9 cells.


TJ-modulating peptide-modified LPN could be a prospective platform for the delivery of chemotherapeutics across the BBB to the brain for the potential treatment of the BM of NSCLC.


Non-small-cell lung cancer (NSCLC) is the most common lung cancer and has become a critical cause of cancer-related deaths globally (Dempke et al. 2015). Patients with NSCLC, especially Asians, are highly associated with epidermal growth factor receptor (EGFR) mutations (Fidler 2015). About 30% of patients with NSCLC develop metastasis in the central nervous system (CNS), such as brain metastases (BM) and leptomeningeal metastases (LM), within the first 2 years after the diagnosis of primary tumors, particularly those harboring EGFR mutation (Dempke et al. 2015; Wei et al. 2019). The systemic administration of EGFR-tyrosine kinase inhibitors (TKIs) is one of the possible treatments (Abdallah and Wong 2018). However, the poor penetration of gefitinib and erlotinib, two first-generation EGFR-TKIs, across the blood–brain barrier (BBB) remains a primary hindrance for this therapeutic regimen (Zeng et al. 2015). The BBB is crucial to regulate CNS homeostasis by maintaining the balance of material exchange between the circulation and the brain (Trabulo et al. 2010). Importantly, the neuroprotective BBB is a dynamic barrier with tight junctions (TJs) to prevent organisms and potential neurotoxins from attacking the CNS (Chen and Liu 2012). The BBB possesses special features, including low paracellular permeability, because of TJs and efflux pump proteins such as P-glycoprotein (On and Miller 2014).

Afatinib (BIBW2992; abbreviated as Afa) is an orally active and second-generation EGFR-TKI. It shows a potent and irreversible effect on suppressing pan-erythroblastic leukemia viral oncogene homolog (ErbB) family, including EGFR, human EGFR 2 (HER2), and HER4 (Hurvitz et al. 2014). Interestingly, evidence has shown the promising results of Afa as a first-line therapy in NSCLC with EGFR mutations and active CNS metastases (Li et al. 2015). Particularly, Afa circumvents resistance to third-generation TKI osimertinib in patients suffering from NSCLC with LM baring-acquired EGFR L858R/L718Q mutation (Rijcken et al. 2007). Although no significant differences are detected in intracranial treatment responses between groups treated with Afa at doses of 30 and 40 mg (Wei et al. 2019), the incidence of rash/acne and paronychia in the group treated with 40 mg of Afa is considerably higher than that in the group treated with 30 mg of Afa (Sasikala et al. 2015). This result suggests the demand of low-dose Afa regimen for reducing the intolerable systemic side effects accompanied with Afa treatment. However, the dose reduction of Afa may lead to a low Afa concentration in the cerebral spinal fluid (CSF) and cause treatment failure against BM (Liu et al. 2017). Afa partially transports across the BBB and penetrates into the BM at low concentrations. Because of its low IC50 against different EGFR variants, Afa may still demonstrate clinical efficacy on limited CNS metastases (Hoffknecht et al. 2015). However, this low Afa concentration seems not enough to treat BM in NSCLC with the EGFR mutation of exon 19 deletion, exon 20 T790M, or exon 21 L858R (Wei et al. 2019). Furthermore, the phase III randomized trial of Afa in patients of trastuzumab-resistant breast cancer with brain metastasis is stopped early through unfavorable risk–benefit analysis (Hurvitz et al. 2014). Moreover, Afa also shows the disadvantage of low solubility in water and commonly used solvents. Hence, it is pivotal to design nanoparticles with multiple functions of modulating the BBB, targeting the cancer focus, and inducing the controlled release of Afa at the site of tumors. The successful development of nanoparticle formulations may provide a potential delivery system for enhancing anticancer efficacy and reducing the associated adverse events of Afa.

Lipid polymeric nanoparticle (LPN) is a potential formulation to deliver therapeutics into the brain by improving common difficulties, such as the BBB penetration, solubility, and toxicity (Sim et al. 2020). It comprises the polymeric core of hydrophobic polylactic-co-glycolic acid (PLGA) coated with lipid layers as the shell (Mandal et al. 2016). This design combines the advantages of both lipophilic polymeric nano-core to encapsulate Afa and amphiphilic lipid-PEG shell, which can be further conjugated with antibodies or peptides for different purposes (Hong et al. 2019). Accordingly, modifying the surface properties of nanoparticles by PEGylation and peptides to disturb TJs of the BBB is a possible strategy for improving drug penetration into the brain (Masserini 2013; Trabulo et al. 2013). Previous studies showing promising results have indicated that FD7 peptide and cyclic (CD) peptide (abbreviated as CCD peptide) are two short peptides that can increase the BBB permeability and have the potential to disrupt the TJs of the BBB (Alaofi et al. 2017, 2016; Bocsik et al. 2016; Herman et al. 2007). FD7 peptide (FDFWITP) was found through screening the phage display peptide library (Herman et al. 2007). It shows the effects on TJ proteins, especially claudins. Claudins are involved in the paracellular ion flux of TJs (Herman et al. 2007). FD7 peptide may bind to the TJ complex or other associated assemblies, particularly claudin 1 or 5 (Herman et al. 2007). This 7-mer peptide enhances the ion permeability and disturbs TJ proteins on the BBB model (Bocsik et al. 2016; Deli 2009; Herman et al. 2007). Intriguingly, FD7 in nontoxic concentrations elicits reversible and more TJ-modulating response in the BBB than in intestinal cells (Bocsik et al. 2016; Deli 2009). CCD peptide (CDTPPVC) is a cyclic derivative from the extracellular 1 (EC1) domain of human E-cadherin (Laksitorini et al. 2015). CCD modulates E-cadherin-mediated cell–cell adhesion and inhibits the adherens junctions of the vascular endothelial cells forming the BBB (Alaofi et al. 2016; Laksitorini et al. 2015). These interactions may generate pores in the intercellular junctions of the BBB and enhance paracellular drug penetration across the BBB (Alaofi et al. 2017, 2016; Laksitorini et al. 2015). CCD has been found to bind to the EC1 domain of human E-cadherin protein as verified with the NMR spectrum (Alaofi et al. 2017). In the present study, the use of Afa-loaded LPN coated with TJ-modulated peptides, such as FD7 and CCD (Afa/LPN-FD7 and Afa/LPN-CCD), as a platform was explored to enhance Afa delivery across the BBB model of mouse brain-derived endothelial cells.3 (bEnd.3) cells to inhibit the growth of human lung adenocarcinoma PC9 cells.


Characterization of Afa encaspulated in LPN (Afa/LPN) and peptife-modified LPN (Afa/LPN-FD7 and Afa/LPN-CCD)

The physicochemical characteristics of Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD are demonstrated in Table 1. A scheme of preparing peptide-conjugated LPNs is shown in Fig. 1. The size of all formulations was 145.26 ± 2.48 to 168.61 ± 4.10 nm (Fig. 2A–C, Table 1). Furthermore, the particle size was evaluated for 28 days and only displayed a slight change (Additional file 1: Figure S1), indicating that the particle size remained constant for at least 28 days. The polydispersity index (PdI) was approximately 0.2, indicating that these nanoparticle formulations were well dispersed and homogeneous (Table 1). The zeta potentials of Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD were − 13.8 ± 0.49, − 10.74 ± 1.42, and − 25.32 ± 1.50 mV, respectively. All the nanoparticles were negatively charged, and the encapsulation efficiency (EE, %) of these formulations was as high as approximately 87% (Table 1). The images of transmission electron microscopy (TEM) showed that the nanoparticles were spherical with an FD7 or CCD peptide-coating shell layer around the PLGA core in which Afa was incorporated (Fig. 2D–F).

Table 1 Characterization of Afa/LPN and peptide-modified LPNs
Fig. 1

Schematic of the formation of Afa-loaded and peptide-modified LPN. Peptides include FD7 or CCD peptide

Fig. 2

Particle size distribution of A Afa/LPN, B Afa/LPN-FD7, and C Afa/LPN-CCD and transmission electron microscopic images of D Afa/LPN, E Afa/LPN-FD7, and F Afa/LPN-CCD

In vitro release of Afa from various LPN formulations

The in vitro release of Afa, Afa loaded in LPN, and FD7- or CCD-conjugated LPN were performed through dialysis in phosphate-buffered saline (PBS, pH 6.5; Fig. 3). This experiment was carried out using the initial Afa concentration of 10 μM in different formulations. Afa-contained formulations were placed in the dialysis device and immersed in PBS at 37 °C for 48 h. The data indicated that free Afa released 72.21% ± 6.15% in the first 1 h. However, the release percentages of Afa from Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD were lower than that of Afa (Fig. 3). After 8 h, 60.26% ± 2.58% and 56.18% ± 1.29% of Afa were released from Afa/LPN-FD7 and Afa/LPN-CCD, respectively, which were remarkably lower than that from Afa/LPN (73.57% ± 4.81%). Afa released from FD7- or CCD-conjugated LPN was below 80% for 48 h in a sustained-release manner. By contrast, free Afa released approximately 95% for 48 h.

Fig. 3

In vitro release of Afa from LPN, LPN-FD7, and LPN-CCD. Afa released from various formulations was performed in the dialysis bag in PBS (pH 6.5) at 37 °C

Cytotoxicity of FD7, CCD, and Afa formulations in bEnd.3 and/or PC9 cells

The cytotoxicity of FD7 and CCD peptides and FD7- and CCD-conjugated LPN on bEnd.3 cells was measured via a sulforhodamine B (SRB) assay (Fig. 4A, B). In Fig. 4A, B, 36 μM FD7 and CCD (marked as #) was the selected concentration for the conjugation of FD7 and CCD with LPN, and the viability of bEnd.3 cells was maintained at approximately 90%. Furthermore, the cytotoxicity of different concentrations of Afa and Afa/LPN was determined in bEnd.3 and PC9 cells for 48 h to identify the suitable concentrations of Afa and Afa/LPN formulations with low toxicity to bEnd.3 cells, respectively (Fig. 4C–E). The viability of bEnd.3 cells decreased to around 70% when Afa concentration reached 1000 nM (1 μM; Fig. 4C). IC50 of Afa in PC9 cells was 1.6 nM (Fig. 4D). Hence, 1.6 nM Afa was used in the following experiments. Particularly, Afa (1.6 nM) did not elicit significant cytotoxic effects on bEnd.3 cells (Fig. 4C). Our results verified that 1.6 nM Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD (marked as #) did not cause noticeable toxic effects on bEnd.3 cells (Fig. 4E). However, Afa (1.6 nM) encapsulated in LPN, LPN-FD7, and LPN-CCD significantly improved the Afa cytotoxicity on PC-9 cells (Fig. 4F).

Fig. 4

Cytotoxicity of FD7, FD7-LPN, CCD, CCD-LPN, and Afa in different formulations on bEnd.3 and PC9 cells. Cytotoxicity of different concentrations of A FD7 and FD7-LPN, B CCD, CCD-LPN, and C Afa on bEnd.3 cells, D Afa on PC9 cells, E Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD on bEnd.3 cells for 48 h, and F Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD on PC9 cells for 48 h. Cell viability was measured via SRB assay. Values are the mean ± S.D. (n = 3). (A, B, E) # represents the concentrations of FD7, CCD, Afa, and Afa/LPN with low cytotoxic effects on bEnd.3 cells, which were used in the following experiments. F *statistical significance at p < 0.05; **p < 0.01; ***p < 0.001

Establishment of the in vitro BBB model

An in vitro BBB model was established by growing bEnd.3 cells on Transwell inserts (Fig. 5A), and the representative image of the BBB model was captured with a Nikon microscope (Fig. 5B). The bEnd.3 cells were stained with SRB, and the morphological characteristics showed that the bEnd.3 monolayer was uniform (Fig. 5B). The integrity of the BBB was investigated by detecting transendothelial electrical resistance (TEER) and permeability (%) levels. bEnd.3 cells were seeded on the insert and cultured for 6 days. The TEER value was detected every day. After the culture of the indicated days, the TEER values increased from 10.23 ± 2.34 Ω·cm2 to 132.24 ± 3.12 Ω·cm2 and reached a plateau after 5 days of incubation (Fig. 5C). Moreover, the relative transendothelial permeability percentage of fluorescein isothiocyanate-conjugated dextran (FITC-dextran; MW 70,000) across the BBB model was 8.52% ± 1.16% compared with that without BBB (blank; 100%; Fig. 5D). In previous studies, the permeability of the BBB model is also evaluated by using FITC-dextran as a paracellular marker (Li et al. 2010; Lin et al. 2016). Hence, the establishment of the BBB model was confirmed via morphological, TEER value, and permeability (%) studies (Fig. 5B–D).

Fig. 5

Establishment of an in vitro blood–brain barrier (BBB) model. A Schematic of the in vitro BBB model; B morphological characteristics of the BBB model stained with sulforhodamine B (SRB). Representative image of an intact cell monolayer was captured under a Nikon light microscope (magnification, 100 ×); C transendothelial electrical resistance (TEER) values were evaluated 6 days after seeding; D permeability of fluorescein isothiocyanate (FITC)-dextran (MW 70,000) across the BBB model on day 5. Values are the mean ± S.D. (n = 3). *p < 0.05: compared with blank via Student’s t-test

Cytotoxicity of Afa in various formulations across the BBB on PC9 cells

The schematic (Fig. 6A) and result in Fig. 6B–D demonstrated the cytotoxicity of different Afa-formulations across the BBB model of Transwell inserts on PC9 cells in the absence or presence of bEnd.3 cells. The cell viability of the blank group was measured when 1.6 nM Afa was transported across the insert without bEnd.3 cells. Afa under this condition decreased the viability of PC9 cells to 66.88% ± 7.65% without the BBB (Fig. 6B). However, the viability of PC9 cells after Afa treatment returned to 85.52% ± 4.19% when the bEnd.3 cells were incubated on the insert, indicating that the BBB model functioned as a barrier to diminish Afa’s delivery into the brain for BM treatment. Interestingly, when Afa was encapsulated in LPN, the viability of PC9 cells decreased to 65.71% ± 3.36%, which was similar to that of the free Afa group without the BBB. Afa-loaded LPN-FD7 and LPN-CCD across the BBB further reduced the viabilities of PC9 cells to 52.89% ± 4.29% and 43.79% ± 3.11%, respectively (Fig. 6B). There were approximately further 33% and 42% decreases on PC9 viability for Afa/LPN-FD7 and Afa/LPN-CCD, respectively, compared to Afa (Fig. 6B), suggesting a superior effect of these two tight junction-modulating peptides on promoting Afa delivery across the BBB model. The cytotoxic effect of Afa/LPN-CCD was higher across the BBB on PC9 cells than that of Afa/LPN-FD7 (Fig. 6B).

Fig. 6

Effect of Afa in various formulations on the cytotoxicity of PC9 cells and the barrier integrity of the BBB model. A Schematic of Afa across the BBB model; B relative cell viability (%) of Afa, Afa/LPN, and peptide-modified LPN across the BBB on PC9 cells. PC9 cells were treated with Afa, Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD with or without the BBB for 48 h. Cell viability was measured via SRB assay. Values are the mean ± S.D. (n = 3). *p < 0.05 compared with the control (CTR), p < 0.05 compared with Afa, p < 0.05 compared with Afa/LPN, and §p < 0.05 compared with Afa/LPN-FD7 through Student’s t-test analysis. C Protein expression levels of apoptosis, including Bax, Bcl-2, and caspase 3, and necroptosis such as RIP1 and 3 were detected with Western blot after the above treatment in PC9 cells

Moreover, the protein expression of PC9 cells after the treatment with various Afa formulations across the bEnd.3 cells for 48 h was examined through Western blot. Bcl-2, an anti-apoptotic protein, was considerably suppressed with the treatments of Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD (Fig. 6C). By contrast, the expression levels of pro-apoptotic proteins, including Bax and caspase-3, were gradually escalated with the treatments of Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD. Free Afa did not cross the insert with bEnd.3 cells very well to display its potency on inducing Bax and inhibiting Bcl-2. However, Afa in LPN, LPN-FD7, and LPN-CCD seemed to considerably cross the BBB model via transcytosis to show the effect on triggering apoptosis (Fig. 6C). Afa encapsulated in LPN, LPN-FD7, and LPN-CCD remarkably induced Bax and suppressed Bcl-2, suggesting the good carrier capability of LPN, especially modified by CCD, on the delivery of Afa across the BBB model. Additionally, necroptosis mediators, such as kinase receptor-interacting protein 1 (RIP1) and RIP3, were upregulated by the treatments of Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD (Fig. 6C). Therefore, these formulations elicited partial cytotoxic effects on PC9 cells via necroptosis.

Cellular uptake mechanisms and barrier function of the BBB model

FD7 and CCD are TJ-modulated peptides (Herman et al. 2007; Laksitorini et al. 2015). Figure 6B shows that the cell growth inhibition of Afa/LPN-FD7 and Afa/LPN-CCD was higher across the BBB in PC9 cells than that of Afa/LPN. However, the cellular uptake mechanisms of Afa/LPN-FD7 and Afa/LPN-CCD formulations on bEnd.3 cells require further investigation. Rhodamine 123 (Rh123) was used as a fluorescent probe of Afa for encapsulation into nanoparticles. The bEnd.3 cells were pretreated with various endocytosis inhibitors, including chlorpromazine (CPZ), 5-(N,N-dimethyl) amiloride (DMA), and nystatin, for 1 h and then incubated with Rh123-loaded LPN-FD7 or LPN-CCD for 3 h. The fluorescence intensity of each group with or without endocytosis inhibitors was detected through flow cytometry, and the means were normalized relative to Rh123/LPN-FD7 (Fig. 7A) or Rh123/LPN-CCD (Fig. 7B). When the cells were pretreated with CPZ (clathrin-mediated endocytosis inhibitor) and nystatin (caveolae-mediated endocytosis inhibitor, the relative fluorescence intensity (%) of Rh123/LPN-FD7 decreased to 67.71% ± 1.63% and 80.83% ± 4.46% compared with that of Rh123/LPN-FD7 without inhibitor (Fig. 7A). Similarly, the pretreatment of cells with CPZ and nystatin decreased the fluorescence intensity of Rh123/LPN-CCD to 78.29% ± 3.27% and 74.23% ± 12.56% compared with that of the Rh123/LPN-CCD group (Fig. 7B). Furthermore, Afa incorporated in the nanoparticles across the BBB might enhance the cytotoxic effect on PC9 cells by decreasing TEER values and increasing the permeability of the bEnd.3 cells compared with those of the free Afa group (Fig. 7C, D). The TEER value of Afa/LPN-FD7 and Afa/LPN-CCD groups decreased to 74.50% ± 3.13% and 65.14% ± 2.08% (Fig. 7C). Afa/LPN-FD7 and Afa/LPN-CCD increased the permeability of FITC-dextran (MW 70,000) across the BBB to 157.49% ± 5.08% and 165.42 ± 7.79%, which significantly differed from those of the control (CTR; 100.00% ± 5.54%), Afa, and Afa/LPN groups (p < 0.05; Fig. 7D). Accordingly, LPN-FD7 and LPN-CCD formulations crossed the BBB not only via the paracellular pathway through the opening of the TJs (Fig. 7C, D) but also via clathrin- and caveolae-mediated transcytosis pathways (Fig. 7A, B). Thus, the enhanced delivery of Afa in LPN-FD7 and LPN-CCD formulations across the bEnd.3 cells caused a higher degree of cytotoxic effect on PC9 cells than that of Afa or Afa/LPN (Fig. 6B). Importantly, after removal of Afa/LPN-FD7 or Afa/LPN-CCD for 3 h, the relative transendothelial permeability percentage of FITC-dextran (MW 70,000) across the BBB model was 8.11% ± 3.28% and 9.27% ± 1.69% compared with that without bEnd.3 cells (blank; 100%). Furthermore, the TEER values were 130.78 ± 4.32 and 131.21 ± 3.86 Ω·cm2 after removal of Afa/LPN-FD7 or Afa/LPN-CCD for 3 h, suggesting a reversible opening of the in vitro BBB barrier and the recovery of integrity of the BBB model.

Fig. 7

Cellular uptake mechanisms and barrier function of the BBB model. A Relative fluorescence intensity (%) of Rh123/LPN-FD7 and B Rh123/LPN-CCD after the treatment of bEnd.3 cells with different endocytosis inhibitors, including CPZ (clathrin-mediated endocytosis inhibitor), DMA (macropinocytosis inhibitor), and nystatin (caveolae-mediated endocytosis inhibitor) for 1 h and then incubated with Rh123-loaded LPN-FD7 or LPN-CCD for 3 h. Values are the mean ± S.D. (n = 3). (A, B): *p < 0.05 compared with A Rh123/LPN-FD7 and B Rh123/LPN-CCD through Student’s t-test analysis, respectively. C TEER after 48 h of the treatment of various Afa formulations on bEnd.3 cells. D Permeability (%) of FITC-dextran (MW 70,000) across the bEnd.3 cells after 48 h of the treatment of various Afa formulations. C, D Values are the mean ± S.D. (n = 3). *p < 0.05 compared with CTR, p < 0.05 compared with Afa, p < 0.05 compared with Afa/LPN, and §p < 0.05 compared with Afa/LPN-FD7 through Student’s t-test analysis

Junctional morphology of bEnd.3 cells through confocal laser microscopy (CLSM) and junctional protein expression of bEnd.3 cells through Western blot

The junctional morphology of bEnd.3 cells was examined through CLSM because FD7 and CCD peptides affect TJs (Herman et al. 2007; Laksitorini et al. 2015). Zonula occludens-1 (ZO-1) and claudin-5 are the major junction proteins of the BBB, so we observed the morphological characteristics of these proteins after the treatment of bEnd.3 cells with Afa, Afa/LPN-FD7, and Afa/LPN-CCD for 48 h. The CLSM images indicated that bEnd.3 cells without treatment exhibited the continuous junctional morphology of ZO-1 and claudin-5 (Fig. 8A). The morphological characteristics of the cells treated with Afa were similar to those of the cells in the control group. Afa did not demonstrate the obvious alteration in the junction proteins of ZO-1 and claudin-5 (Fig. 8). Nevertheless, the junctions of ZO-1 and claudin-5 were discontinuous after the cells were treated with Afa/LPN-FD7 and Afa/LPN-CCD (Fig. 8A). The junctional proteins in the Afa/LPN-CCD group were apparently disturbed (Fig. 8A). This phenomenon indicated that the nanoparticles conjugated with TJ-modulated peptides might disrupt the arrangement of junctional proteins, such as ZO-1 and claudin-5, consequently enhancing the Afa transport across the BBB. Additionally, the junctional protein expression of bEnd.3 cells was also evaluated after the treatment with different Afa formulations through Western blot. However, Afa/LPN-FD7 and Afa/LPN-CCD did not exhibit an obvious effect on the junctional protein expression of bEnd.3 cells, including ZO-1, claudin-5, and E-cadherin; this result suggested the perturbing effect of Afa/LPN-FD7 and Afa/LPN-CCD on the arrangement of TJ-associated proteins instead of suppressing the junctional protein levels (Fig. 8).

Fig. 8

Effect of peptide-modified LPN on the junctional morphology and protein expression of bEnd.3 cells. A Immunostaining of the TJ proteins ZO-1 and claudin-5 after the treatment of bEnd.3 cells with Afa, Afa/LPN-FD7, and Afa/LPN-CCD for 48 h observed through confocal laser scanning microscopy (magnification, 1500x). Green: ZO-1. Red: claudin-5. Blue: the cell nuclei stained with DAPI. B Protein expression levels of claudin-5, ZO-1, and E-cadherin were detected through Western blot after the above treatment in bEnd.3 cells


Afa was approved by the FDA in 2013 as a first-line treatment for patients with EGFR-specific mutations, including exon 19 deletions or exon 21 (L858R) substitutions (Lee et al. 2014). The covalent bond between the acrylamide of Afa and the cysteine residue within the active site of the intracellular tyrosine kinase domain of EGFR, HER2, and HER4 caused the irreversible inhibition of downstream signaling pathways, effectively inhibited cell growth, and induced apoptosis in NSCLC (D'Arcangelo and Hirsch 2014; Li et al. 2008). A recent investigation has indicated that three first-line EGFR-TKIs, such as gefitinib, erlotinib, and Afa, demonstrate a comparable treatment efficacy based on the results of progression-free survival and overall survival in patients with EGFR-mutated NSCLC and brain metastases (Rijcken et al. 2005). Nevertheless, limited information was reported for the effective brain concentration and the intracranial response rates of Afa (Hoffknecht et al. 2015). For the effective treatment of NSCLC with EGFR mutation, Afa at the dose of 40 mg daily was commonly recommended by the European Medicines Agency (Yang et al. 2016). Although Afa has been generally regarded as predictable adverse events, such as cutaneous and gastrointestinal (GI) symptoms (Hirsh 2015), it causes some serious side effects, including grades 3–4 diarrhea (14%), rash or acne (16%), and paronychia (11%) (Yang et al. 2015). The dose-limiting GI toxicities and the detrimental effects due to the irreversible inhibition of EGFR by Afa may halt the further clinical application of Afa at a high dose. However, the dose reduction of Afa because of the intolerable side effects accompanied with high-dose Afa often causes the low concentration of Afa in the CSF and the treatment failure against BM (Liu et al. 2017). Interestingly, the surface modification of nanoparticles to disturb TJs of the BBB may enhance drug delivery to the brain (Masserini 2013). In the current study, two nanoparticle Afa formulations, including Afa/LPN-FD7 and Afa/LPN-CCD, were successfully developed. These two nanoformulations exhibited a homogeneous shape, acceptable particle size and surface potential, and a sustained-release profile (Table 1, Figs. 2 and 3). FD7 and CCD peptides increase the BBB permeability with the potential to interrupt TJs of the BBB (Alaofi et al. 2017; Herman et al. 2007). In a previous research, a BBB-permeable peptide is used to increase the BBB permeability as an effective strategy for the treatment of CNS inflammatory diseases (Lim et al. 2015). Recently, a neuronal damage inhibitor was linked to the cell-penetrating Tat peptide for modulating the BBB adherence, thereby promoting the neuronal uptake and permeation of this inhibitor across the BBB (Zhan et al. 2011). Our finding indicated that peptides, such as FD7 and CCD, and Afa-loaded nanoparticles, including Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD, did not cause a significant cytotoxic effect on bEnd.3 cells (Fig. 4). Afa/LPN-FD7 and Afa/LPN-CCD across the BBB model of bEnd.3 cells enhanced the cytotoxic effect of Afa on PC9 cells. FD7 and CCD perturbed TJ proteins, such as claudin 5 and ZO-1 (Fig. 8A), decreased the TEER (Fig. 7C) of bEnd.3 cells, and increased the permeability (%) of FITC-dextran across the bEnd.3 cells (Fig. 7D). This result suggested that these formulations might cross the BBB via paracellular pathway by disrupting the interactions of TJ proteins, diminishing the TJ-associated barrier functions without affecting the protein expression of TJs (Fig. 8B). Furthermore, Afa/LPN-FD7 and Afa/LPN-CCD were partially transported across the BBB via transcellular pathway by clathrin- and caveolae-mediated transcytosis (Fig. 7AB). These findings revealed the effective activation of both paracellular and transcellular pathways by these LPN modified with TJ-interrupted peptides to facilitate Afa delivery across the BBB for promoting the cytotoxic effect on PC9 cells (Fig. 6B). Afa/LPN-CCD exhibited more negative zeta potential, possibly due to two carboxylate groups in the side chains of CCD, thus displaying more repulsion and smaller particle size than that of Afa/LPN-FD7 (Table 1). CCD, a cyclic peptide from human E-cadherin (Laksitorini et al. 2015), might condense itself into compact structure to generate pores in the intercellular junctions of the BBB (Alaofi et al. 2017) and thus demonstrated stronger perturbing effect on the BBB integrity and function than that of FD7. Hence, Afa/LPN-CCD showed more enhancement on cytotoxicity of Afa against PC9 cells than that of Afa/LPN-FD7 (Fig. 6B). The schematic of the transport of Afa/LPN-FD7 and Afa/LPN-CCD across the BBB model of bEnd.3 cells via paracellular and transcellular pathways to release Afa is shown in Fig. 9.

Fig. 9

Schematic of the delivery of Afa/LPN-FD7 and Afa/LPN-CCD across the BBB model of bEnd.3 cells via paracellular and transcellular pathways to transport Afa


Collectively, Afa encapsulated in LPN coated with TJ-modulating FD7 or CCD can improve the cytotoxicity of Afa across the BBB on PC9 cells via paracellular and transcellular pathways. TJ-modulating peptide-modified LPN can be a potential platform for the delivery of chemotherapeutics across the BBB to the brain for the treatment of brain tumors or the BM of tumors such as NSCLC.

Materials and methods


Afa was obtained from MedChem Express, Monmouth, NJ, USA. FD7 and CCD were purchased from Kelowna Biotech (Taipei, Taiwan) at > 95% purity. Lecithin was purchased from Alfa Aesar (Heysham, LNH, Kingdom) and DSPE-PEG2000-NHS was obtained from Nanocs Inc. (Boston, MA, USA). All cell culture medium and reagents were bought from Promega (Madison, WI, USA), Invitrogen (Carlsbad, CA, USA), Gibco BRL (Grand Island, NY, USA), or Hyclone (Logan, UT, USA). All other chemical reagents were obtained from either Merck (Darmstadt, Germany) or Sigma-Aldrich.

Preparation of Afa/LPN and peptide-conjugated Afa/LPN

Peptide-linked lipids were initially produced by dispersing FD7 or CCD peptides and DSPE-PEG2000-NHS at a molar ratio of 1:1 in chloroform and methanol (1:1) for 24 h at room temperature. After forming the peptide-lipid conjugates, the organic solvent was evaporated and the residue was then dialyzed against water by dialysis bag (molecular weight cutoff at 0.5–1 kDa; Spectrum Laboratories, CA, USA). The purified peptide-linked lipids were collected by freeze-drying. The conjugation has been confirmed using a MALDI-TOF mass spectrometer (MALDI-TOF MS).

Afa-loaded nanoparticles were prepared by an o/w emulsion method. mPEG-DSPE (2 mg/ml) and lecithin (2 mg/ml) were mixed into PBS buffer to prepare aqueous dispersion. To prepare 1.6 nM Afa/LPN, 6.4 µl of 1 μM afatinib in DMSO was added to 4 ml PLGA (2 mg/ml; 50:50 LA:GA; MW 35,000–45,000) in acetone solution. Afa in PLGA solution was then cautiously added drop by drop into aqueous lipid dispersion. The resulting o/w emulsion was sonicated for 10 min using a bath sonicator at 50% amplitude. The organic solvent was then removed by constant evaporation under vacuum at room temperature. The preparation of Afa/LPN-FD7 and Afa/LPN-CCD was the same as the preparation of Afa/LPN, but mPEG2000-DSPE was replaced with the individual peptide-conjugated lipid.

Characterization of Afa-loaded nanoparticles

Afa-contained nanoparticles dispersion was centrifuged at 25,200g and 4 °C through an ultracentrifuge filter (Amicon®, MW: 10 kDa). Afa in the supernatant was detected by HPLC. The HPLC system consists of a pump (PM1110; Hitachi, Tokyo, Japan), an autosampler (Primaide 1210; Hitachi), a reverse C18 column (Phenomenex), and a L2420 UV detector (Hitachi). The mobile phase was prepared by mixing water, acetonitrile, and methanol (55:25:20 v/v). The solution was degassed by a sonicator before detection. The UV detection was carried out at wavelength 254 nm and flow rate was set at 1.0 mL/min. Records were analyzed from three independent experiments. Encapsulation efficiency (EE %) was computated by the following formula:

$$EE\% {\text{ }} = {\text{ }}\left[ {\left( {{W_e}-{\text{ }}{W_s}} \right)/{\text{ }}{W_e}} \right] \times 100\%,$$

where We is the weight of added Afa and Ws is the weight of Afa in the supernatant.

The size distribution and zeta potential of nanoparticles were monitored by Zetasizer Nano ZS90 dynamic light scattering system (Malvern Instruments Ltd., Malvern, Worcestershire, UK) at 25 °C and a detection angle of 90° for LPN dispersion. The resulting particle size distribution was plotted as the intensity (%) of LPN versus size in diameter (nm). Data were analyzed from four individual measurements and computed by a cumulant method to automatically obtain polydispersity index using Zetasizer family software v7.11.

The morphology of nanoparticles was captured under transmission electron microscopy (JEM-2000EX, JEOL, Ltd., Tokyo, Japan). The samples were diluted with double-distilled water and dropped on the grid for 1 min. The samples were immersed in 1% phosphotungstic acid. The excess of solution was sucked from the edge with a filter paper and then dried in the air at room temperature.

In vitro release of Afa from nanoparticles

The release study of Afa from Afa dispersion, Afa/LPN, Afa/LPN-FD7, and Afa/LPN-CCD was implemented by dialysis method. 1 mL of Afa-contained formulation at Afa equivalent concentration of 10 µM was suspended into a dialysis bag (3.5 ~ 5 kDa MWCO membrane, Spectrum, Inc., Rancho Dominguez, CA, USA). The dialysis medium was 100 mL PBS (pH = 7.4) with stirring at 18 g and 37 °C. At time points of 0.5, 1, 2, 4, 8, 12, 24, and 48 h, 0.1 mL sample was removed from the dialyzer and the same volume of PBS was supplemented into the dialyzer to maintain the same volume of solution. Afa concentration in each sample was analyzed by HPLC. The cumulative release of Afa was then computated.

Culture of PC-9 cells and bEnd.3 cells and cytotoxicity evaluation by SRB assay

bEnd.3 cells were grown in Dulbecco′s Modified Eagle Medium (DMEM) nourished with F-12 (DMEM/F12; Gibco), 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin. PC9 cells are human lung adenocarcinoma cells with EGFR exon 19 deletion. Cells were grown in Roswell Park Memorial Institute-1640 Medium (RPMI-1640; Hyclone) supplemented with 10% FBS and 1% penicillin/streptomycin. Cell lines were all maintained in the incubator with 5% CO2 at 37 °C. Other cell culture reagents were puchased from Corning. bEnd.3 and PC9 cells were seeded at density of 2 × 104 cells/well and 4 × 103 cells/well in 96-well flat transparent plates and cultured for 24 h. Different concentrations of Afa, FD7, or CCD in free or the corresponding LPN formulations were added to the medium and cultured for 48 h, as indicated in Figs. 4 and 6. The cultured medium was then removed and the cytotoxicity was determined by SRB assay. Briefly, after adding 0.04% SRB into each well for 10 min, the individual well was washed three times with 1% acetic acid. After drying the wells at room temperature for 24 h, 10 mM Tris base was added to the separate well. The absorbance was measured at 540 nm by a Tecan microplate reader.

Establishment of in vitro BBB model: morphology of barrier integrity

bEnd.3 cells were seeded at a density of 2 × 104 cells/insert on ThinCert™ cell culture inserts (Greiner Bio-One, Germany). Cells were cultured up to 6 days and the intergriy of monolayers were examined to verify if the BBB model was suitable for the following experiments. The bEnd.3 cells were stained with SRB and morphology of the BBB model was detected. The images of mophology was observed under a Nikon Diaphot 300 Inverted Tissue Culture Microscope (Nikon, Tokyo, Japan).

Verification of BBB model: TEER measurement

bEnd.3 cells were seeded at a density of 2 × 104 cells/insert on ThinCert™ cell culture inserts. After culture up to 6 days, the monolayers were formed for the following experiments as a BBB model. Two electrodes of Millicell ERS-2 Epithelial Volt-Ohm Meter (Millipore, Billerica, MA, USA) were mounted in the medium of the upper insert and lower well, respectively. The electrodes can detect the resistance values across the inserts with or without the bEnd.3 monolayers. TEER was calculated by the following formula:

$$TEER = \left( {{R_{total}} - R_{blank}^{}} \right) \times A\left( {\Omega \times c{m^2}} \right)$$

where Rtotal is the resistance acorss the cell layers on permeable inserts, Rblank is the resistance of peremable inserts without cells and A is the surface area of insert membrane (0.336 cm2).

Verification of BBB model: permeability measurement

After incubation, the lower wells were replaced with 0.6 mL HBSS buffer. 0.1 mL FITC-dextran (0.09 mg/mL; MW 70,000; a paracellular marker) were then added to the upper inserts. After incubation of these cells in the dark at 37 °C for 1 h, 0.1 mL solution of lower well was collected. Fluorescence intensity of FITC-dextran was measured at excitation 494 nm and emission at 521 nm using a Tecan Infinite 200® PRO multimode microplate reader (Männedorf, Switzerland). The relative percentage of permeability was calculated by normalizing the blank values (without cell seeding on the inserts; Fig. 5D) or CTR (no treatment; Fig. 7D) as 100%.

Cytotoxicity of Afa-formulations across the BBB on PC9 cells by SRB assay

bEnd.3 cells were seeded at a density of 2 × 104 cells/inser on the upper chamber of ThinCert™ 24-well inserts. PC9 cells were seed at a density of 2 × 104 cells/well on the lower plates of 24 well and cultured for 24 h. bEnd.3 cells were cultured for 5 days and treatments of different Afa-formulations were added into the medium in the upper chamber. After treatments of these cells for 48 h, the upper inserts were removed. The cytotoxicity was examined by SRB assay. Cell viability % was normalized relatively to the control by dividing the absorbance values of the treated cells with that of cells of medium control.

Western blot assay

bEnd.3 and PC9 cells (2 × 105 cells/dish) were incubated in the 6 cm dish, and different Afa-formulations were added and cultured for 48 h. After lysis of cells by radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Beverly, MA, USA), the proteins were extracted and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Proteins (30 μg/lane) were run by 8–13.5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and moved to a polyvinylidene difluoride membrane at 80 V for 120 min. After blocking, the membranes were incubated with primary antibodies against Bax, Bcl-2, Casp-3, RIP-1, RIP-3, Claudin 5, ZO-5, E-cadherin, and ß-actin overnight at 4 °C. Then, the samples were immersed with horseradish peroxidase-conjugated secondary IgG at room temperature for 1 h. The relative protein expression was examined using a chemiluminescent ImageQuant LAS 4000 Scanner of GE Healthcare (Piscataway, NJ, USA).

Endocytic uptake mechanisms of Rh123-loaded nanoparticles

bEnd.3 cells were seed in the 24-well plate overnight and pretreated with different endocytosis inhibitors, including chlorpromazine hydrochloride (CPZ; 10 µM, clathrin-mediated endocytosis inhibitor), 5-(N,N-dimethyl) amiloride hydrochloride (DMA; 10 µg/mL, micropinocytosis inhibitor), nystatin (Nys; 20 µg/mL, and caveolae-mediated endocytosis inhibitor) for 30 min, and then treated with Rh123-loaded nanoparticles for 3 h. Rh123 is a cell-permeant dye with green fluorescence (Ex/Em = 488/534 nm) (Petrat et al. 2003). It is used as a fluorescent probe of Afa for incorporation into nanoparticles without cytotoxic effects. After treatments, the cells were collected, centrifuged at 300g and 4 °C and re-suspended in cold PBS. The harvested cells were monitored by FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA). Fluorescence was collected through a FL1 filter (515–545 nm) for Rh123 and fluorescence signals were transferred into a logarithmic scale. Data processing and analysis were conducted by BD FACStation™ software (BD Biosciences). Each group was carried out in triplicate.

Effect of Afa/nanoparticles on immunofluorescence staining of TJ proteins by confocal laser scanning microscope (CLSM)

Immunostaining was performed for TJ proteins, including claudin-5 and ZO-1. After treatments, cells were washed with phosphate buffer (PBS), and fixed with mixture of ice cold acetone and methanol for 10 min at room temperature. After blocking with 3% bovine serum albumin in PBS, cells were incubated with primary antibodies of mouse anti-claudin-5 (Invitrogen, Camarillo, CA, USA) and rabbit anti-ZO-1 (Invitrogen, Camarillo, CA, USA) overnight. The cells were detected with secondary antibodies of FITC-labeled anti-rabbit (Jackson Immunoresearch, West Grove, PA, USA) and Texas red-labeled anti-mouse (Jackson Immunoresearch, West Grove, PA, USA) for 1 h. DAPI was used to stain cell nuclei. After mounting the samples, these images were visualized by a confocal laser scanning microscope (Olympus FV10i, Olympus America Inc., Center Valley, PA, USA).

Statistical analysis

Experimental data were expressed as the mean ± SD and analyzed by Student’s t-test. p < 0.05 was considered statistically significant.

Availability of data and materials

All data and materials are included in the manuscript.



Non-small-cell lung cancer


Epidermal growth factor receptor


Central nervous system


Brain metastases


Leptomeningeal metastases


Epidermal growth factor receptor-tyrosine kinase inhibitors


Epidermal blood–brain barrier


Tight junctions


Human epidermal growth factor receptor




Pan-erythroblastic leukemia viral oncogene homolog


Cerebral spinal fluid


Lipid polymeric nanoparticle


Polylactic-co-glycolic acid


Cyclic (CD) peptide


Afa-loaded LPN coated with FD7


Afa-loaded LPN coated with CCD

bEnd.3 cells:

Brain-derived endothelial cells.3


Human lung adenocarcinoma PC9 cells


Transmission electron microscopy


Encapsulation efficiency


Polydispersity index


Sulforhodamine B


Transendothelial electrical resistance


Fetal bovine serum


Fluorescein isothiocyanate-conjugated dextran


Kinase receptor-interacting protein


Rhodamine 123


5-(N,N-Dimethyl) amiloride




Confocal laser scanning microscopy


Zonula occludens-1




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We thank Professor Chuen-Mao Yang (Program for Biotech Pharmaceutical Industry, China Medical University) for kindly providing the bEnd.3 cells.


This research was funded by National Yang Ming Chiao Tung University and Cheng Hsin General Hospital (CY10935; CY11004), Ministry of Science and Technology of Taiwan (MOST 107–2320-B-010–015-MY3; MOST 106–2320-B-010–005), Veterans General Hospitals and University System of Taiwan Joint Research Program (VGHUST110-G2-2–2; VGHUST109-V7-3–2), and Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang Ming Chiao Tung University.

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YL conceived, designed and interpreted the experiments as well as wrote the original and revised manuscript; SH, CC, and CW performed the experiments and analyzed the data; HL and AML conceived and interpreted the experiments; YL, HL, and AML contributed reagents/materials/analysis tools. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yu-Li Lo or Anya Maan-Yuh Lin.

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The authors declare no conflict of interest. The funders had no involvement in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Supplementary Information

Additional file 1: Figure S1.

Stability of afatinib (Afa) in LPN-FD7 and LPN-CCD for 28 days. Afa/LPN-FD7 and Afa/LPN-CCD were stored at 4 °C and PBS of pH7.4 for 28 days. The size was measured by Zetasizer. The values are the mean ± standard error (SD). For each group, n = 3.

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Lo, YL., Lin, HC., Hong, ST. et al. Lipid polymeric nanoparticles modified with tight junction-modulating peptides promote afatinib delivery across a blood–brain barrier model. Cancer Nano 12, 13 (2021).

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  • Afatinib
  • Blood–brain barrier
  • Lipid polymeric nanoparticle
  • Tight junction-modulating peptide
  • Lung cancer
  • Brain metastasis